mouse anti delta fosb antibody  (Proteintech)

Journal: The Journal of Clinical Investigation

Article Title: Chloride homeostasis dysfunction drives hyperactivation of corticotropin-releasing factor-expressing neurons in the amygdala in stress-induced hypertension

doi: 10.1172/JCI195536

Figure Lengend Snippet: ( A and B ) Changes in MAP ( A ) and HR ( B ) measured by radiotelemetry before, during and after CUMS in WKY rats, BHRs and age-matched unstressed WKY rats and BHRs ( n = 6 rats in each group). Data are expressed as means ± SEM. * P < 0.05 for CUMS BHR versus unstressed BHRs, # P < 0.05 for CUMS BHR versus CUMS WKY, ^ P < 0.05 for CUMS WKY versus unstressed WKY. Repeated measures of 2-way ANOVA with Tukey’s post hoc test. ( C ) Representative immunofluorescence images showing CRF immunopositive neurons (green), delta-FosB immunopositive neurons (red), and CRF and delta-FosB double-positive neurons in the CeA of WKY, CUMS WKY, BHR, and CUMS BHR group of rats ( n = 5 rats in each group). ( D and E ) Summary data showing the numbers of CRF-positive neurons ( D ) and delta-FosB positive neurons ( E ) in the CeA of these 4 groups. ( F ) The percentage of CRF and delta-FosB double-positive neurons in the CeA of these 4 groups ( n = 5 rats in each group). Data are expressed as means ± SEM. ** P < 0.01, *** P < 0.001. One-way ANOVA with Tukey’s multiple comparison tests.

Article Snippet: Subsequently, the sections were incubated with a mouse anti-delta-FosB antibody (1:50, #sc-398595), a rabbit anti-CRF antibody (1:100, #A1122, Abclonal) or a rabbit anti-NKCC1 antibody (1:100, #13884, Proteintech) overnight at 4°C.

Techniques: Immunofluorescence, Comparison

Journal: Neurobiology of Stress

Article Title: Repeated vagus nerve stimulation produces anxiolytic effects via upregulation of AMPAR function in centrolateral amygdala of male rats

doi: 10.1016/j.ynstr.2022.100453

Figure Lengend Snippet: rVNS increases excitatory neurotransmission in CeL and inhibitory neurotransmission in CeM. (A) The timeline of the experiments. (B) Expression of c-Fos positive cells (green) and ΔFosB positive cells (red) in the centrolateral amygdala (CeL) and centromedial amygdala (CeM) of rVNS-treated rats compared with sham rats. Arrows indicated co-labeled cells. Scale bar, 50 μm. (C) rVNS treatment increased c-Fos and ΔFosB expression in the CeL, but no difference in CeM. n = 12 slices from 3 rats per group. (D) Representative traces of AMPAR-mediated mEPSCs in the CeL from sham and rVNS-treated rats. Scale bar, 20 pA and 2 s. (E) Cumulative probabilities of mEPSCs amplitude and statistics of mEPSCs amplitude for representative cells from each group. rVNS increased the amplitude of mEPSCs by ∼20% relative to sham-treated rat. n = 13 cells for sham, n = 11 cells for rVNS. (F) Cumulative probabilities of mEPSCs frequency and statistics of mEPSCs frequency for representative cells from each group. The frequency of mEPSCs was significantly increased in rVNS-treated rat. n = 13 cells for sham, n = 11 cells for rVNS. (G) Representative mIPSCs recording in the CeL neurons from sham and rVNS-treated rats. Scale bar, 50 pA and 2 s. (H) Cumulative probabilities of mIPSCs amplitude and statistics of mIPSCs amplitude for representative cells from each group. rVNS had no effect on the amplitude of mIPSCs in rVNS-treated rats. n = 17 cells for each group. (I) Cumulative probabilities of mIPSCs frequency and statistics of mIPSCs frequency for representative cells from each group. The frequency of mIPSCs was unaltered in rVNS-treated rats. n = 17 cells for each group. (J) Representative mIPSCs traces in CeM neurons from sham and rVNS group. Scale bars, 50 pA and 2 s. (K) Cumulative probabilities of mIPSCs amplitude and statistics of mIPSCs amplitude for representative cells from each group. The amplitude of mIPSCs recorded in CeM neuron was increased significantly in rVNS-treated rats. n = 13 cells for sham, n = 15 cells for rVNS. (L) Cumulative probabilities of mIPSCs frequency and statistics of mIPSCs frequency for representative cells from each group. rVNS significantly increased the frequency of mIPSCs. n = 13 cells for sham, n = 15 cells for rVNS. Data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01; n.s., not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The primary antibodies included: rabbit anti-c-Fos (1:1000 dilution; Abcam, ab208942, Cambridge, UK) and mouse anti-ΔFosB antibody (1:1000 dilution; Cell Signaling Technology, 14695, Denvars, MA, USA).

Techniques: Expressing, Labeling